Interleukin 31 (IL-31) is a cytokine with a four-helix bundle structure. It has structural and functional similarities to IL-6, Oncostatin M, LIF, and Cardiotrophin-1. The human IL-31 gene encodes a precursor of 164 amino acids that includes a 23 amino acid signal peptide and a 141 amino acid mature protein. IL-31 has human and murine homologues with a sequence identity of 24 percent in the mature region. IL-31 is mainly associated with activated T cells and is preferentially expressed by type 2 helper T cells (Th2). The IL-31 signaling pathway is activated by the heterodimeric receptor complex consisting of an IL-31RA (also known as GPL and GLMR) protein and an Oncostatin M receptor (OSM R-beta). Keratinocytes express the IL-31 receptor in a permanent manner and can be induced by IFN gamma on monocytes. GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulates immune responses that are linked with skin physiology and inflammatory skin diseases. Studies have shown that IL31 induces severe pruritis (itching) and dermatitis in transgenic mice. Human Interleukin-31/IL-31 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin-31/IL-31 Recombinant Protein (RPES2376)
Product Code:
RPES2376
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Interleukin-31, IL-31, IL31
Accession:
Q6EBC2
Sequence:
Ser24-Thr164
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-31 is produced by our E.coli expression system and the target gene encoding Ser24-Thr164 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
15.8 kDa
AP Mol Mass:
15 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
