IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They regulate a variety of cellular activities, including cell proliferation, differentiation, and survival/apoptosis, as well as several pathological processes including viral infections and autoimmune diseases. Cytokines are produced by a variety of cells in the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems in response to a variety of stimuli. However, IL-21 is expressed in activated CD4+ T-cells but not in CD8+ T-cells, B-cells, or monocytes. IL-21 may help promote the switch between innate and adaptive immunity. IL-21 has been used to treat asthmatic reactions. It has been demonstrated to reduce pro-inflammatory cytokines and IgE levels in a mouse model of rhinitis (nasal passage inflammation). Human Interleukin-21/IL-21 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin-21/IL-21 Recombinant Protein (RPES1853)
Product Code:
RPES1853
Size:
5µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Interleukin-21,IL-21, Za11, IL21
Accession:
Q9HBE4-1
Sequence:
Gln 23-Ser 155
Fusion tag:
Activity:
1. Measured by its ability to bind human IL-21R in a functional ELISA.2. Measured by its ability to induce Interferon-gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.4-2 ng/ml.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human IL-21 isoform 1 (Q9HBE4-1) (Gln 23-Ser 155) was expressed and purified, with an initial Met.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
15.6 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
