The IL-17 family include IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F. The family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes.
Product Name:
Human IL7A&IL7F Heterodimer Recombinant Protein (RPES1979)
Product Code:
RPES1979
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
IL-17A/F Heterodimer,IL-17A&IL-17F Heterodimer
Accession:
Q16552&Q96PD4
Sequence:
Ile20-Ala155&Arg31-Gln163
Fusion tag:
C-6His
Activity:
Measured by its ability to induce IL-6 secretion by NIH?3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 93 pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-17A/Interleukin-17F Heterodimer is produced by our Mammalian expression system and the target gene encoding Ile20-Ala155&Arg31-Gln163 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
43.2 kDa
AP Mol Mass:
15-18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
