IL-1 receptor–like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interlukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described, and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage pro-inflammatory function. IL33 is a specific ligand of ST2L and induces production of Th2 cytokines.
Product Name:
Human IL1RL1/ST2 Recombinant Protein (RPES2055)
Product Code:
RPES2055
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
DER4,FIT-1,IL1RL1,IL33R,IL?1 ,R4,ST2,ST2L,ST2V,T1
Accession:
NP_003847.2
Sequence:
Met 1-Phe 328
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 µg/ml (100 ?l/well) can bind human IL1RL1 / Fc Chimera with a linear range of 0.31-5 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human IL1RL1 isoform 2 (NP_003847.2) (Met 1-Phe 328) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
67.1 kDa
AP Mol Mass:
85-95 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
