Interleukin-17 receptor (IL-17R); also known as Interleukin-17 receptor A (IL-17RA) and CD217 antigen (CD217); is a cytokine receptor which binds interleukin 17. IL-17R/IL-17RA (CD217) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17R/IL-17RA (CD217) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor IL-17RA play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors; this receptor likely has a multimeric structure. Defects in IL-17R/IL-17RA (CD217) are the cause of familial candidiasis type 5 (CANDF5). CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections; selective against Candida. It is characterized by persistent and/or recurrent infections of the skin; nails and mucous membranes caused by organisms of the genus Candida; mainly Candida albicans.
Product Name:
Human IL17RA Recombinant Protein (RPES1394)
Product Code:
RPES1394
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CANDF5,CD217,CDw217,hIL-17R,IL-17RA,IL17R
Accession:
NP_055154.3
Sequence:
Met 1-Trp 320
Fusion tag:
C-His
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL17A at 2 µg/ml (100 ?l/well) can bind biotinylated human IL17RA with a linear range of 1.28-32 ng/ml.2. Measured by its ability to inhibit IL-17-induced IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 0.03-0.12 µg/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of human IL17RA (NP_055154.3) (Met 1-Trp 320) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
35 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
