R-spondin-2; also known as RSPO2; synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta- catenin-dependent pathway; possibly via a direct interaction with Wnt proteins; and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells; consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice; they exhibited greater metastatic activity than cells derived from Wnt1 tumors.
Product Name:
Human IL17BR/IL17RB Recombinant Protein (RPES4590)
Product Code:
RPES4590
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CRL4,EVI27,IL17BR,IL17RH1
Accession:
NP_061195.2
Sequence:
Met 1-Gly289
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-Fc at 10µg/mL (100?L/well) can bind biotinylated human Fc-IL25.The EC50 of human Fc-IL25 is 4.5-10.4ng/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human IL17BR (NP_061195.2) (Met1-Gly289) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
56.9 kDa
AP Mol Mass:
67 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
