Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Product Name:
Human IL10RB/IL10R2 Recombinant Protein (RPES4917)
Product Code:
RPES4917
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CDW210B,CRF2-4,CRFB4,D21S58,D21S66,IL-10R2,Interleukin-10 receptor subunit beta(IL10RB),Cytokine receptor class-II member 4,Cytokine receptor family 2 member 4,Interleukin-10 receptor subunit 2
Accession:
NP_000619.3
Sequence:
Met 1-Ser 220
Fusion tag:
C-His & Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human IL28B at 20 µg/ml (100 ?l/well) can bind human IL10RB-Fc with a linear ranger of 3.125-25 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
DNA sequence encoding the extracellular domain (Met 1-Ser 220) of human IL10R? (NP_000619.3) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
51.7 kDa
AP Mol Mass:
75-85 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
