Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine that contributes to cortical inflammation, cell growth and tissue regeneration. The IL1 superfamily is made up of three species: IL1A (IL1 alpha), IL1B (IL1 beta), and the receptor antagonist, IL1Ra. Interleukin (IL)-1 has been connected to Th1/Th2 immune responses in clinical studies, and it has been implicated in the development of successful pregnancy. Interleukin (IL) 1 gene polymorphisms that are proinflammatory and promote higher levels of IL-1beta activity raise the risk for hypochlorhydria and distal gastric cancer. Possible associations between the IL1B polymorphisms and SSc susceptibility have been identified. Finally, the IL2-384-G allele may be a sign of SSc's circumscribed form (SSc). Human IL beta/IL1B Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IL beta/IL1B Recombinant Protein (RPES1518)
Product Code:
RPES1518
Size:
5µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
IL-1,IL-1 beta,IL-1B,IL1-BETA,IL1F2
Accession:
NP_000567.1
Sequence:
Ala 117-Ser 269
Fusion tag:
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 µg/mL (100?l/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.3. Measured by its binding ability in a functional ELISA.4. Immobilized human IL1B at 10 µg/mL (100 ?l/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 µg/mL.5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human IL1? (NP_000567.1) (Ala 117-Ser 269) was expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
