The cytokines of the IL-6/GCSF/MGF family are glycoproteins with four conserved cysteine residues arranged in two disulfide bonds and approximately 170 to 180 amino acids in length. Their structure is very similar to other interleukins, and they have a compact, globular fold that is stabilized by the two disulfide bonds. The structure contains a 4 alpha-helix bundle with a left-handed twist and helices that are anti-parallel, with 2 overhand bonds, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is crucial to the molecule's biological function. Interleukin-6 (IL-6) is a proinflammatory and immunoregulatory cytokine produced by various cells. In vitro, interleukin-6 has been found to slow the growth of early-stage melanoma cells and stimulate their growth in advanced-stage melanoma cells. Human IL-6/Interleukin-6 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IL-6/Interleukin-6 Recombinant Protein (RPES1357)
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 20-100pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-6 is produced by our E.coli expression system and the target gene encoding Val30-Met212 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
20.9 kDa
AP Mol Mass:
20 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
