Interleukin-6 (IL-6) is a multifunctional alpha helical cytokine that controls cell growth and differentiation of a wide range of tissues, particularly the immune response and acute phase reactions. The IL-6 protein is produced in various cell types, including T cells and macrophages, as a phosphorylated and glycosylated molecule that varies in length. IL-6 exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is involved in a wide range of processes including hematopoiesis, bone metabolism, and cancer progression. It has been characterized as an important component in the switch from innate to acquired immunity. Human IL-6/Interleukin-6 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IL-6/Interleukin-6 Recombinant Protein (RPES1238)
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 µg/ml (100 ?l/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL.2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.75-3 ng/mL. The specific activity of Recombinant Human IL-6 is approximately 0.35 - 105 IU/µg.3. Measured by its binding ability in a functional ELISA. 2.Immobilized human IL6 at 10µg/mL (100?L/well) can bind human IL6R-His, the EC50 of human IL6R-His is 0.1-0.5 µg/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val 30-Met 212) was expressed, with an initial Met at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.3 kDa
AP Mol Mass:
20.3 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
