Interleukin-22 receptor subunit alpha-2 (IL-22RA2); also known as interleukin-22-binding protein (IL-22BP); is a subunit of the receptor for interleukin 22. IL-22BP belongs to the type I I cytokine receptor family and contains 3 fibronectin type-III domains. IL-22BP/IL-22RA2 is expressed in a range of tissues; including those in the digestive; female reproductive; and immune systems. It is expressed in placenta; spleen; breast; skin and lung. It is also detected in intestinal tract; testis; brain; heart and thymus. The dominant cell types expressing IL-22BP/IL-22RA2 were mononuclear cells and epithelium. IL-22BP/IL-22RA2 may play an important role as an IL-22 antagonist in the regulation of inflammatory responses. Interleukin-22 (IL-22) is a member of IL-10 family. It is produced by T cells and induces the production of acute-phase reactants. IL-22 plays important roles in immune response through activation of the STAT 3 signal transduction pathway. Two types of IL-22-binding receptor have been discovered; a membrane-bound receptor and a soluble receptor.
Product Name:
Human IL-22BP/IL22RA2 Recombinant Protein (RPES2226)
Immobilized IL22BP-His at 10 µg/ml (100 ?l/well) can bind biotinylated IL22; The EC50 of biotinylated IL22 is 1.78-4.14 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human IL22RA2 isoform 2 (NP_851826.1) (Met 1-Pro 231) was expressed; fused with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
26.1 kDa
AP Mol Mass:
50-55 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
