Recombinant Human IgG1 Protein (RPCB2072)

Product Name:	Recombinant Human IgG1 Protein
SKU:	RPCB2072
Size:	50µg
Tag:	C-His
Reactivity:	Human
Expression Host:	HEK293 cells

Protein Description:	High quality, high purity and low endotoxin recombinant Recombinant Human IgG1 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:	<0.1EU/μg
Purity:	> 95% by SDS-PAGE.
Formulation:	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:	3500

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

Storage:	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:	P01857

	Recombinant Human IgG1 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions ，showing single bands at 35-40 kDa and 70-100 kDa, respectively.
	Immobilized Human Fc-gamma RII-a(CD32a) at 1μg/mL (100 μL/well) can bind IgG1 Fc with a linear range of 0.156-3.47μg/mL.