Insulin-Like Growth Factor II (IGF2) belongs to the insulin family of polypeptide growth factors that is involved in development and growth. This family includes molecules structurally similar to proinsulin that have a greater sequence similarity. The IGF2 is expressed only from the paternally inherited allele, and it is thought to be released by the liver and circulated throughout the blood. IGF-2 has been shown to stimulate the proliferation and survival of a variety of cell types, including muscle, bone, and cartilage tissue in vitro. IGF2 is influenced by placental lactogen and may play a role in fetal development. Human IGF-II/IGF2 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IGF-II/IGF2 Recombinant Protein (RPES1602)
Product Code:
RPES1602
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Insulin-Like Growth Factor II, IGF-II, Somatomedin-A, IGF2, PP1446
Accession:
P01344
Sequence:
Ala25-Glu91
Fusion tag:
Activity:
Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells. The ED50 for this effect is 15.7 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Long Insulin-Like Growth Factor II is produced by our E.coli expression system and the target gene encoding Ala25-Glu91 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
8.9 kDa
AP Mol Mass:
15 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 5mM Hac, pH ~3.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
