Heat shock protein 90 (90 kDa heat-shock protein, HSP90) is a cellular chaperone that aids in the movement of proteins. It's a highly adaptable protein that is important in the stress response and routine homeostatic management. HSP90 interacts with 'client proteins,' such as protein kinases, transcription factors, and other proteins, and either stabilizes and activates them or directs them for proteasomal degradation. HSP90 has a broad range of activities in signal transduction, chromatin remodelling, and epigenetic regulation, development, and morphological evolution. HSP90 is a dimer in which the N-terminal region undergoes conformational changes driven by ATP binding and hydrolysis at the end of the molecule. Disruption of HSP90 results in client protein degradation and, in some instances, cell death. In stressful situations, HSP90 protects its client proteins and the cell against cellular stressors such as in cancer cells. Tumor cells, in particular, several oncoproteins function as HSP90 client proteins and to maintain their malignancy, they need greater HSP90 activity than ordinary cells. Because of this, Hsp90 has become a leading target in the field of cancer therapy. Human HSP90AA1/HSP90 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human HSP90AA1/HSP90 Recombinant Protein (RPES1310)
A DNA sequence encoding the human HSP90 isoform 2 (NP_005339.3) C-terminal segment, corresponding to amino acid sequence (Glu 535-Asp 732) was expressed and purified, with two additonal aa (Gly & Pro) at the N terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
22.6 kDa
AP Mol Mass:
24 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
