Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is most apparent during S phase and then decreases significantly as cell growth slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, restricting DNA accessibility to cellular machineries that require a template. The histones are essential in transcription regulation, DNA repair, DNA reproduction and chromosomal stability. The accessibility of DNA is controlled by a variety of posttranslational modifications of histones, also known as histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. The structure of the histone octamer is as follows: The four core histones (H2A, H2B, H3, and H4) make up an octamer that has approximately 146 bp of DNA wrapped around it. Chromatin fibers are further compacted via the connection of a linker histone, H1, with DNA between the nucleosomes to build higher order chromatin structures. Histone H3.1/HIST1H3A/H3FA Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Histone H3.1/HIST1H3A/H3FA Recombinant Protein (RPES2514)
A DNA sequence encoding the native human HIST1H3A (NP_003520.1) (Met1-Ala136) was expressed. Human and Mouse HIST1H3A sequences are identical.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
15.5 kDa
AP Mol Mass:
15.5 kDa
Formulation:
Lyophilized from sterile 2mM ?-Mercaptoethanol
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
