The histones are a large, complex family of highly conserved basic proteins that bind chromosomal DNA into nucleosomes. There are numerous subtype diversities: H1, H2A, H2B, and H3 or H4. Histone modifications have increasingly been shown to be key players in the control of chromatin states and dynamics as well as gene expression. As a result, histone modifications and enzymatic machineries that establish them are key regulators of cell growth, differentiation, flexibility, and malignancy. However, extracellular histones are a double-edged sword since they may also harm host tissue and cause death. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. The protein H2a, also known as histone H2A (HIST3H2A), is a component of histones. Covalent proteolysis and modification of histones is essential for chromatin dynamics and transcription. One example of such modification is ubiquitination, which mainly occurs on histones H2A and H2B. E3 ubiquitin ligase complex is specific for histone H2A (HIST3H2A). In HeLa cells, reducing the expression of Ring2 promotes ubiquitination and degradation of H2A. DNA damage induces monoubiquitylation of histone H2A (HIST3H2A) in the vicinity of DNA lesions. Human HIST3H2A/Histone H2A Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human HIST3H2A/Histone H2A Recombinant Protein (RPES5097)
Product Code:
RPES5097
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
MGC3165
Accession:
NP_254280.1
Sequence:
Met 1-Lys 130
Fusion tag:
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human HIST3H2A (NP_254280.1) (Met 1-Lys 130) was expressed and purified.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
14.2 kDa
AP Mol Mass:
14.2 kDa
Formulation:
Lyophilized from sterile 20mM ?-Mercaptoethanol, pH 6.9
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
