HDAC4 (histone deacetylase 4), belongs to class II of the histone deacetylase/acuc/apha family. A family of enzymes known as histone deacetylases (HDACs) are closely associated with sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. They are not autonomous in the sense that they do not function alone, but rather as components of larger multiprotein complexes such as pRb-E2F and mSin3A, which control important transcriptional regulators. HDACs are divided into three categories: classes 1, 2, and 4, which are Zn2+-dependent enzymes. HDACs are present in all cells and may be found within the nucleus or cytosol. Protein-protein interactions and the class to which they belong determine their subcellular localization. These enzymes have been linked to cell growth arrest, differentiation and death, resulting in a lot of interest in HDAC inhibitors as potential anti-neoplastic medicines. The HDAC4 protein has histone deacetylase activity and represses transcription when bound to a promoter. It is not a DNA-binding transcription factor, but one that activates transcription factors MEF2C and MEF2D indirectly. HDAC4 seems to interact in a multiprotein complex with RbAp48 and HDAC3. Human HDAC4 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human HDAC4 Recombinant Protein (RPES5195)
Product Code:
RPES5195
Size:
20µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
AHO3,BDMR,HA6116,HD4,HDAC-4,HDAC-A,HDACA
Accession:
NP_006028.2
Sequence:
Met612-Leu1084
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human HDAC4 (Met612-Leu1084) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
50.9 kDa
AP Mol Mass:
51 kDa
Formulation:
Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10%gly
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
