Members of the glypican-related integral membrane proteoglycan family (GRIPS) have a core protein linked to the cytoplasmic membrane via a glycosyl phosphatidylinositol link. GPC3 appears to be involved in cell division and growth control. GPC3 binds to and inhibits CD26's dipeptidyl peptidase activity, causing cell death. GRIPS is a key component in the cell cycle, DNA repair, and chromatin maintenance. GPC3 has been linked to a number of ailments, including Simpson-Golabi-Behmel Syndrome, Type 1 and Wilms tumor 1. Development VEGF signaling via VEGFR2 and glycosaminoglycan metabolism are two pathways influenced by GPC3. Human Glypican 3/GPC3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Glypican 3/GPC3 Recombinant Protein (RPES2429)
Immobilized Human FGFb(Cat: PKSH032437) at 2µg/ml(100 ?l/well) can bind Human GPC3-His. The ED50 of Human GPC3-His is 0.23 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Glypican-3 is produced by our Mammalian expression system and the target geneencoding Gln25-His559 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
61.6 kDa
AP Mol Mass:
70-150&38 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
