Glial Cell Line-Derived Neurotrophic Factor Family Receptor ?-1 (GDNFR?1) is a glycosylphosphatidylinositol (GPI) linked cell surface protein belonging to GDNF-family receptor ? subtype which consists of at least four members. GFR?1and GFR?2 are the cognate co-receptor for the neurotrophic factor neurturin mediating the NRTN-induced autophosphorylation and activation of the RET tyrosine kinase receptor. Soluble GFR?s released enzymatically from the cell surface by phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR?1, can also bind with high affinity to GDNF and trigger the activation of Ret tyrosine kinase. Human GFR?1 shares 93% amino acid identity with mouse GFR?1.The expression of the various GFR?s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR?s in mediating the activities of the GDNF family of neurotrophic factors.
Immobilized Human GDNF(Cat: PKSH032488) at 1.5µg/ml(100 ?l/well) can bind Human GFRA1-His. The ED50 of Human GFRA1-His is 0.468 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human GFRA1 is produced by our Mammalian expression system and the target gene encoding Asp25-Lys429 is expressed with a 6His tag at the C-terminus.
Purity:
> 90% as determined by reducing SDS-PAGE.
Mol Mass:
46.28 kDa
AP Mol Mass:
60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
