Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a N-terminal nucleophile aminotransferase that acts as the rate-limiting enzyme for glucose entry into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The entire length of human GFAT contains 1 glutamine amidotransferase type-2 domain that catalyzes the transfer of amide nitrogen from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains present in several phosphosugar isomerases and phosphosugar binding proteins. Two isoforms of GFAT have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system. Human GFPT1/GFAT Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human GFPT1/GFAT Recombinant Protein (RPES3946)
Product Code:
RPES3946
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
CMSTA1,GFA,GFAT,GFAT1,GFAT1m,GFPT,GFPT1L,MSLG
Accession:
AAA58502.1
Sequence:
Gln 332-Glu 699
Fusion tag:
Endotoxin:
Please contact us for more information.
Protein Construction:
The sequence corresponding to amino acids (Gln 332-Glu 699) of human GFAT (AAA58502.1) was expressed and purified with two amino acids (Gly & Pro) at the N-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
41.5 kDa
AP Mol Mass:
41.5 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4, 10% glycerol
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
