The Galectin family of lectins consists of beta-galactoside binding proteins with homologous carbohydrate recognition domains (CRDs). They also have hemagglutination activity, which is due to their bivalent carbohydrate binding capabilities. Intracellularly and extracellularly, galectins are active. They have diverse effects on many cellular functions including adhesion; migration; polarity; chemotaxis; proliferation; apoptosis; and differentiation. Galectins may be involved in a wide range of diseases, including autoimmune disorders; allergy responses; inflammation; tumor cell metastasis; atherosclerosis; and diabetic issues. The prototype galectins (1; 2; 5; 7; 10; 11, 13; 14) are molecules containing one CRD and form either a monomer or a noncovalent homodimer. Galectin3 chimera galectins (Galectin3) with a single CRD linked to a nonlectin domain, and Galectins (4; 6; 8; 9; 12), which include two CRDs connected by a linker peptide. Galectins lack a known signal peptide and are instead found in the cytosolic compartments where they fulfill intracellular roles. Galectins, however, can also be secreted via one or more yet unknown nonclassical secreting pathways to operate extracellularly. Individuals within the galectin family have diverse tissue distributions and varying carbohydrate-binding preferences. Each family member may preferentially bind to a unique subset of cell surface glycoproteins. Human Galectin-3/LGALS3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Galectin-3/LGALS3 Recombinant Protein (RPES4654)
Measured by its ability to agglutinate human red blood cells.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Galectin-3 is produced by our E.coli expression system and the target gene encoding Ala2-Ile250 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
26.0 kDa
AP Mol Mass:
30 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; 2mM DTT;pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
