Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.
Product Name:
Human G-CSFR/CD114 Recombinant Protein (RPES0154)
Product Code:
RPES0154
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD114,CSF3R,G-CSF R,GCSFR
Accession:
NP_000751.1
Sequence:
Met 1-Pro 621
Fusion tag:
C-Fc
Activity:
Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells.The ED50 for this ettect is typically 2-20 ng/ml in the presence of 0.125ng/ml of recombinant human GCSF.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Pro 621) of human G-CSF receptor (NP_000751.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
93.3 kDa
AP Mol Mass:
120-130 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
