Fibronectin1 (FN1) is a secreted protein and contains 12 fibronectin type-I domains; fibronectin type-II domains and 16 fibronectin type-III domains. Recombinant human fibronectin component is a protein of about 63 kilodaltons with a central cell-binding domain, a high affinity heparin-binding domain II, and CS1 site in the continuously spliced III CS region of human fibronectin. A VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors bind to a heparin binding domain II, which co-locate the cell and the virus vector on NovoNectin. This technique increases the density of both cells and vectors, as well as making it easier for genes to be transferred in the end. Human Fibronectin/FN Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Fibronectin/FN Recombinant Protein (RPES4158)
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
62.7 kDa
AP Mol Mass:
60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 12.5 mM Sodium Citrate; 1.25% Sucrose; pH 6.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
