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Recombinant Human Fibroblast Activation Protein α/FAP Protein (Fc Tag) (RPES8386)

SKU:
RPES8386
Size:
20μg
Tag:
C-Fc
Reactivity:
Human
Expression Host:
Mammalian
Accession:
Q12884
€289

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Recombinant Human Fibroblast Activation Protein α/FAP Protein (Fc Tag)
SKU:RPES8386
Size:20μg
Tag:C-Fc
Reactivity:Human
Expression Host:Mammalian
Accession:Q12884
Sequence:Leu26-Asp760
Calculated Molecular Weight:105.7 kDa
Observed Molecular Weight:110 kDa
Synonyms:170 kDa melanoma membrane bound gelatinase, 170 kDa melanoma membrane-bound gelatinase, DPPIV, DPPIVA, FAPA, Fibroblast activation protein alpha, Integral membrane serine protease, SEPR, Seprase, SIMP, FAP, FAPalpha
Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method
Purity:> 90% as determined by reducing SDS-PAGE.

Seprase, also known as 17 KD melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to thepeptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 17-KD dimer, consisting of two 97-KD subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-associated molecules. In association with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

Bio-activity:Not validated for activity
Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

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