Fibroblast growth factors (FGF) are a group of heparin-binding proteins produced by cells that promote cell proliferation and specialization in a variety of tissues. FGFs have a wide range of biological functions in both vivo and in vitro, including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. In cell culture media, the cells of human embryonic stem cell (hESC) cultures require FGF basic (also known as bFGF) to remain undifferentiated and pluripotent. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a necessary component of stem cell culture media for keeping cells in an undifferentiated state. Because FGF basic is sensitive; frequent media changes are required. The thermostable FGF basic is a two-day media change option that requires no media changes over the weekend. The thermostable FGF basic was more stable than FGF basic in biochemistry tests; and it maintained cell growth, pluripotency, and differentiation potential with a two-day feeding regimen. Human FGF-2/FGF basic/FGFb Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human FGF-2/FGF basic/FGFb Recombinant Protein (RPES4411)
Recombinant Human FGF-2(Q65I;C96S;N111G) is produced by our E.coli expression system and the target gene encoding Met1-Ser155 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
17.2 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris;200mM NaCl; pH7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
