Fibroblast growth factors (FGF) are a group of heparin-binding proteins produced by cells that promote cell proliferation and specialization in a variety of tissues. It has been shown that FGFs have a wide range of biological roles in both vivo and in vitro, including mitosis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures in cell culture media require FGF basic (also known as bFGF) to remain undifferentiated and pluripotent. The thermostable FGF basic was created for improved maintenance in culture medium; its biological function has not been altered. For keeping cells in an undifferentiated state, FGF basic is a necessary component of stem cell culture media. Because FGF basic is sensitive to media changes, frequent replacements are necessary. The thermostable FGF basic is a two-day media change option that does not require any media changes during the weekend. In biochemistry activities, the thermostable FGF basic was more stable than FGF basic; and it maintained cell growth, pluripotency, and differentiation potential with a two-day feeding regimen. Human FGF-2/FGF basic/FGFb Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human FGF-2/FGF basic/FGFb Recombinant Protein (RPES4433)
Recombinant Human FGF-2(K128N) is produced by our E.coli expression system and the target gene encoding Met1-Ser155(Lus128Asn) is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
17.2 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris;200mM NaCl; pH7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
