Recombinant Human Ephrin-A5/EFNA5 Protein (RPCB0330)

Product Name:	Recombinant Human Ephrin-A5/EFNA5 Protein
SKU:	RPCB0330
Size:	10µg
Tag:	C-hFc&His
Reactivity:	Human
Expression Host:	HEK293 cells

Protein Description:	High quality, high purity and low endotoxin recombinant Recombinant Human Ephrin-A5/EFNA5 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:	< 0.1 EU/μg of the protein by LAL method.
Purity:	> 92% by SDS-PAGE.
Formulation:	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Gene ID:	1946

Ephrin-A5 also known as EFNA5, is a member of the Ephrin family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Storage:	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:	P52803

	Recombinant Human Ephrin- A5/EFNA5 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 50-60 kDa.
	Immobilized Human EPHA2 at 0.5μg/mL (100 μL/well) can bind Human EFNA5 with a linear range of 0.02-0.67ng/mL.