Description
Product Name: | Recombinant Human Dihydropyrimidinase/DPYS Protein |
SKU: | RPCB2036 |
Size: | 100µg |
Tag: | No tag |
Reactivity: | Human |
Expression Host: | Baculovirus-Insect Cells |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Dihydropyrimidinase/DPYS Protein , tested reactivity in Baculovirus-Insect Cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 1.0 EU/μg of the protein by LAL method. |
Purity: | > 95% by SDS-PAGE. |
Formulation: | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Gene ID: | 1807 |
DPYS, also known as dihydropyrimidinase, belongs to the DHOase family, hydantoinase/dihydropyrimidinase subfamily. DPYS catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. It can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. DPYS is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Swiss-Prot: | Q14117 |