The enzyme oligosaccharyltransferase (oligotropholipidprotein glycosyltransferase) (DDOST), or 48-kDa subunit (OST48), is a typical type I membrane topology, with a large luminal domain, a hydrophobic transmembrane domain, and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric aggregate of three endoplasmic reticulum-bound type I transmembrane proteins: ribophorin I (RI), ribophorin II (RII), and OST48. It is a non-glycoprotein, non-ribophorin protein that does not elicit an immunological response. OST48 was discovered to be a membrane protein, with the majority of the polypeptide embedded inside the ER lumen. OST48 does not show significant amino acid sequence homology to either ribophorin I or II. Only the luminal domain of RI, it was discovered, contains ER retention information. The dilysine motif in OST48 acts as an ER localization motif since OST48 with the two lysine residues replaced by serine (OST48ss) is no longer retained in the ER and instead appears at the plasma membrane. Human DDOST/OST48 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human DDOST/OST48 Recombinant Protein (RPES5024)
Product Code:
RPES5024
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
AGER1,CDG1R,OKSWcl45,OST,OST48,WBP1
Accession:
P39656-1
Sequence:
Ser 43-Pro 427
Fusion tag:
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human DDOST (P39656-1) extracellular domain (Ser 43-Pro 427) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
42.7 kDa
AP Mol Mass:
46 kDa
Formulation:
Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
