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Human DDOST/OST48 Recombinant Protein (RPES5024)

SKU:
RPES5024
Product Type:
Recombinant Protein
Species:
Human
$1,750
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Description

system_update_altDatasheet

Human DDOST/OST48 Recombinant Protein

The enzyme oligosaccharyltransferase (oligotropholipidprotein glycosyltransferase) (DDOST), or 48-kDa subunit (OST48), is a typical type I membrane topology, with a large luminal domain, a hydrophobic transmembrane domain, and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric aggregate of three endoplasmic reticulum-bound type I transmembrane proteins: ribophorin I (RI), ribophorin II (RII), and OST48. It is a non-glycoprotein, non-ribophorin protein that does not elicit an immunological response. OST48 was discovered to be a membrane protein, with the majority of the polypeptide embedded inside the ER lumen. OST48 does not show significant amino acid sequence homology to either ribophorin I or II. Only the luminal domain of RI, it was discovered, contains ER retention information. The dilysine motif in OST48 acts as an ER localization motif since OST48 with the two lysine residues replaced by serine (OST48ss) is no longer retained in the ER and instead appears at the plasma membrane. Human DDOST/OST48 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.