The cystatin superfamily comprises a variety of natural cysteine protease inhibitors found in a wide range of organisms and is divided into three categories. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin that is primarily expressed in hematopoietic cells and has the highest expression levels in monocytes, dendritic cells, and certain types of T-cells. Cystatin F mRNA is also increased during dendritic cell maturation, suggesting that cystatin F has a specific role in immune activation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. Cystatin F and its homologues have been discovered in human cancer cell lines created from malignant tumors, suggesting a new approach to diagnose and prevent certain human carcinomas metastasis. Human Cystatin 7/CST7 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Cystatin 7/CST7 Recombinant Protein (RPES1089)
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of the human Cystatin F (NP_003641.3) (Met 1-His 145) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
15.2 kDa
AP Mol Mass:
25 kDa
Formulation:
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
