Cytotoxic T-lymphocyte protein 4; also known as CTLA4 and CD152; is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members; CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role; CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular; recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells; termed regulatory T cells (Treg).Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Proteins CTLA4 / CD152 Immune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human CTLA4/CD152 Recombinant Protein (RPES3194)
Product Code:
RPES3194
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
ALPS5,CD,CD152,CELIAC3,CTLA-4,GRD4,GSE,IDDM12
Accession:
NP_005205.2
Sequence:
Met 1-Phe 162
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human CTLA4 at 10 µg/mL (100 ?l/well) can bind human B7-1/Fc; The EC50 of human B7-1/Fc is 14 ng/mL.3. Immobilized human CTLA4 at 10 µg/mL (100 ?l/well) can bind human B7-2/Fch; The EC50 of human B7-2/Fch is 53 ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
15 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
