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Recombinant Human Collagen I alpha 1/COL1A1 Protein (RPCB1108)

SKU:
RPCB1108
Size:
10µg
Tag:
C-His
Reactivity:
Human
Expression Host:
HEK293 cells
$203

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Recombinant Human Collagen I alpha 1/COL1A1 Protein
SKU:RPCB1108
Size:10µg
Tag:C-His
Reactivity:Human
Expression Host:HEK293 cells
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Human Collagen I alpha 1/COL1A1 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 0.1 EU/μg
Formulation:Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:1277

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides . The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues . Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described . This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P02452
Product ImageRecombinant Human Collagen I alpha 1/COL1A1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-35 kDa.

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