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Recombinant Human Coagulation factor III/Tissue factor/CD142 Protein (RPCB0251)

SKU:
RPCB0251
Size:
20µg
Tag:
C-His
Reactivity:
Human
Expression Host:
HEK293 cells
€279

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Recombinant Human Coagulation factor III/Tissue factor/CD142 Protein
SKU:RPCB0251
Size:20µg
Tag:C-His
Reactivity:Human
Expression Host:HEK293 cells
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Human Coagulation factor III/Tissue factor/CD142 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 0.1 EU/μg of the protein by LAL method.
Purity:> 95% by SDS-PAGE.
Formulation:Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Gene ID:2152

The protein is coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P13726
Product ImageRecombinant Human Coagulation factor III/CD142 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 37-45 kDa.
Product ImageImmobilized Human CD142 at 1μg/mL (100 μL/well) can bind Human Tissue Factor Rabbit mAb with a linear range of 0.98-61.71 ng/mL.

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