Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.
Product Name:
Human CFL2/cofilin 2/ADF Recombinant Protein (RPES1497)
Product Code:
RPES1497
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
NEM7
Accession:
Q9Y281-1
Sequence:
Ala 2-Leu 166
Fusion tag:
N-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human CFL2 (Q9Y281-1) (Ala 2-Leu 166) was expressed, with a polyhistidine tag at the N-terminus.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
20.4 kDa
AP Mol Mass:
21 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
