FCAR, also called Fc?RI or CD89, is a type I transmembrane receptor for Fc region of IgA which is the most abundant immunoglobulin in mucosal areas but is only the second most common antibody isotype in serum. This receptor is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, especially phagocytes located in mucosal areas. Upon ligand IgA binding, Fc?RI associates with the FcR ? signaling molecule bearing the immunoreceptor tyrosine-based activation motif (ITAM) through a unique charge-based mechanism and triggers multiple cell-mediated immune responses. It has been reported that Fc RI is a dual-function receptor that can mediate both inflammatory and anti-inflammatory responses depending on the type of interaction with its ligand. Sustained aggregation of FCAR results in activation of target-cell functions such as antigen presentation and cytokine release. In contrast, Monomeric targeting with serum IgA or with a variety of anti-Fc?RI Fab fragments triggers an inhibitory response and additionally induces apoptosis. Fc?RI thus play an fundamental role in preventing tumor development and growth, as well as in controlling inflammation.
Product Name:
Human CD89/FCAR Recombinant Protein (RPES3376)
Product Code:
RPES3376
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Immunoglobulin Alpha Fc Receptor, IgA Fc Receptor, CD89, FCAR,CTB-61M7.2,FcalphaRI,FCAR,XXbac-BPG230H20.5
Accession:
NP_001991.1
Sequence:
Met 1-Asn 227
Fusion tag:
C-His
Activity:
Measured by its ability to bind human IgA in functional Elisa.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the pro form of human FCAR extracellular domain (NP_001991.1) (Met 1-Asn 227) was expressed with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
25 kDa
AP Mol Mass:
45-50 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
