CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein; which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues; as well as ovarian tumors; being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets; CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human CD47 Recombinant Protein (RPES5243)
Product Code:
RPES5243
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
IAP,MER6,OA3
Accession:
NP_942088.1
Sequence:
Met 1-Pro139
Fusion tag:
C-Fc
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 µg/ml (100 ?l/well) can bind human CD47-Fc; The EC50 of human CD47-Fc is 10.1-23.5 ng/ml.2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His at 10 µg/ml (100 ?l/well) can bind human CD47-Fc; The EC50 of human CD47-Fc is 0.05-0.13 µg/ml.3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His at 10 µg/ml (100 ?l/well) can bind human CD47-Fc; The EC50 of human CD47-Fc is 0.58-1.34 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
40.7 kDa
AP Mol Mass:
48-60 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
