CD3E & CD3G Heterodimer Recombinant Protein (RPES2797)
- SKU:
- RPES2797
- Product Type:
- Recombinant Protein
- Species:
- Human
Frequently bought together:
Description
system_update_altDatasheet
Product Name: | CD3E & CD3G Heterodimer Recombinant Protein (RPES2797) |
Product Code: | RPES2797 |
Size: | 20µg |
Species: | Human |
Expressed Host: | HEK293 Cells |
Synonyms: | CD3E, CD3G Heterodimer,IMD18,T3E,TCRE |
Accession: | NP_000724.1&NP_000064.1 |
Sequence: | Met 1-Asp126&Met1-Ser116 |
Fusion tag: | |
Activity: | Measured by its ability to bind OKT3-mIgG2a in a functional ELISA. |
Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
Protein Construction: | A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. |
Purity: | > 95 % as determined by reducing SDS-PAGE. |
Mol Mass: | 77.2 (40.1+37.1) kDa |
AP Mol Mass: | 42-47 kDa |
Formulation: | Lyophilized from sterile PBS, pH 7.4. |
Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |