Fc?RIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of Fc?RIIA, Fc?RIIB, and Fc?RIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc?RII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc?RIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of Fc?RIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of Fc?RIIB on dendritic cells inhibits maturation and blocks cell activation. Fc?RIIB may also be a target for monoclonal antibody therapy for malignancies. Fc?RIIB plays an important negative-regulating role through modulating the signals from activation receptors.
Product Name:
Human CD32b/FCGR2B Recombinant Protein (RPES4216)
Product Code:
RPES4216
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b, IgG Fc Receptor II-b, CDw32, Fc-Gamma RII-b, Fc-Gamma-RIIb, FcRII-b, CD32, FCGR2B, FCG2, IGFR2
Accession:
NP_001002274.1
Sequence:
Thr43-Pro217
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor II-B is produced by our Mammalian expression system and the target gene encoding Thr43-Pro217 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.6 kDa
AP Mol Mass:
27 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
