Fc?RIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of Fc?RIIA, Fc?RIIB, and Fc?RIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc?RII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc?RIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of Fc?RIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of Fc?RIIB on dendritic cells inhibits maturation and blocks cell activation. Fc?RIIB may also be a target for monoclonal antibody therapy for malignancies. Fc?RIIB plays an important negative-regulating role through modulating the signals from activation receptors.
Product Name:
Human CD32b/FCGR2B Recombinant Protein (RPES4980)
Product Code:
RPES4980
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b, IgG Fc Receptor II-b, CDw32, Fc-Gamma RII-b, Fc-Gamma-RIIb, FcRII-b, CD32, FCGR2B, FCG2, IGFR2
Accession:
NP_001002274.1
Sequence:
Ala 46-Ile 224
Fusion tag:
C-His-AVI, Biotinylated
Activity:
Measured by its binding ability in a functional ELISA.Immobilized Human IgG1 at 10 µg/ml (100 ?l/well) can bind Human CD32b. The EC50 of Human CD32b is 1.6 - 3.7 µg/ml.2. Labeling ratio of biotin to protein: 0.5
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
24 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
