The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.
Product Name:
Human CD32b/FCGR2B Recombinant Protein (RPES5001)
Product Code:
RPES5001
Size:
50µg
Species:
Human
Expressed Host:
CHO Cells
Synonyms:
Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b, IgG Fc Receptor II-b, CDw32, Fc-Gamma RII-b, Fc-Gamma-RIIb, FcRII-b, CD32, FCGR2B, FCG2, IGFR2
Accession:
P31994-1
Sequence:
Ala46-Pro217
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.Immobilized human sCD32b-His (CHO) at 10 µg/ml (100 ?l/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.18-0.42 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding (Ala46-Pro217) of human CD32b (P31994-1) was expressed,with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
20.8 kDa
AP Mol Mass:
29 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
