CD27; also known as TNFRSF7; is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage; and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer; CD27 has been shown to play roles in lymphoid proliferation; differentiation; and apoptosis.It has important role in generation of T cell immunity; and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule; the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity; and meanwhile; plays a key role in regulating B-cell differentiation; activation and immunoglobulin synthesis.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: ICC Antibodies Immune Checkpoint Detection: FCM AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human CD27/TNFRSF7 Recombinant Protein (RPES0603)
Product Code:
RPES0603
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
S152,S152. LPFS2,S152.LPFS2,T14,TNFRSF7,Tp55
Accession:
P26842
Sequence:
Met 1-Ile192
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA.Immobilized human CD27-his at 10 µg/mL (100 ?l/well) can bind biotinylated human CD70-Fc; The EC50 of biotinylated human CD70-Fc is 95-150 ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CD27 (P26842) (Met1-Ile192) was expressed; fused with the Fc region of rabbit IgG at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
44.4 kDa
AP Mol Mass:
63 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
