CD247, also known as CD3-ZETA, belongs to the CD3Z/FCER1G family. It contains 3 ITAM domains. As a -cell receptor zeta, CD247 forms the T-cell receptor-CD3 complex together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and –epsilon. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. Defects in CD247 can cause immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Product Name:
Human CD247/CD3-ZETA Recombinant Protein (RPES0981)
Product Code:
RPES0981
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
CD3-ZETA,CD3H,CD3Q,CD3Z,IMD25,T3Z,TCRZ
Accession:
P20963-1
Sequence:
Arg52-Arg164
Fusion tag:
N-GST
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human CD247 (P20963-1) (Arg52-Arg164) was fused with the GST tag at the N-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
40.2 kDa
AP Mol Mass:
28-43 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
