The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD21, also known as Complement component (3d / Epstein Barr virus) receptor 2 and CR2, is a member of the CD system and is a protein involved in complement system. CD21 is present on all mature B-cells and some T-cells and follicular dendritic cells. CD21 on mature B-cells form a complex called the B cell receptor complex with two other membrane proteins, CD19 and CD81. CD21 has a function in the complement system through serving as the cellular receper specific for ligands such as C3 and C4 which can be attached to foreign macromolecules in order to remove or uptake them. This results in B-cells having enhanced response to the antigen.
Product Name:
Human CD21/CR2/C3DR Recombinant Protein (RPES0763)
Product Code:
RPES0763
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
C3DR,CD21,CR,CVID7,SLEB9
Accession:
P20023-1
Sequence:
Met 1-Arg 971
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human CD21 at 2 µg/ml (100 ?l/well) can bind biotinylated human CD9. The EC50 of biotinylated human CD9 is 1.76 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CD21 isoform A (P20023-1) extracellular domain (Met 1-Arg 971) was expressed, with a carboxy-terminal polyhistidine tag.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
106 kDa
AP Mol Mass:
110-120 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
