Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.
Product Name:
Human CD208/LAMP3/DC-LAMP Recombinant Protein (RPES2263)
Product Code:
RPES2263
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD208,DC LAMP,DC-LAMP,DCLAMP,LAMP,LAMP-3,TSC403
Accession:
NP_055213.2
Sequence:
Lys 28-Thr 381
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human LAMP3 (NP_055213.2) (Lys 28-Thr 381) with a carboxy-terminal polyhistidine tag was expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
39 kDa
AP Mol Mass:
70-90 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
