CD1B contains 1 Ig-like (immunoglobulin-like) domain and belongs to the CD1 family. CD1 family members are transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. During protein synthesis and maturation, they bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1B localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.. It is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. CD1B is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Product Name:
Human CD1B/CD1A Recombinant Protein (RPES5078)
Product Code:
RPES5078
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD1,CD1A,CD1B,R1
Accession:
NP_001755
Sequence:
Met 1-Ser 303
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CD1B (NP_001755) (Met1-Ser 303) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 88 % as determined by reducing SDS-PAGE.
Mol Mass:
58.6 kDa
AP Mol Mass:
59-69 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
