The Fc receptor with low affinity for IgG (FCGR3; or CD16) is encoded by 2 nearly identical genes; FCGR3A and FCGR3B; resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A; it is also known as CD16a; encodes a transmembrane protein expressed on activated monocytes/macrophages; natural killer (NK) cells; and a subset of T cells.CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells; independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells; independent of antibody ligation.
Product Name:
Human CD16a/FCGR3A Recombinant Protein (RPES0192)
Product Code:
RPES0192
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A, CD16a Antigen, Fc-Gamma RIII-Alpha, Fc-Gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc Receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3,CD16,CD16A
Accession:
P08637-1
Sequence:
Met 1-Gln 208
Fusion tag:
C-His & AVI
Activity:
1. Measured by its ability to bind recombinant human IgG1 in a functional ELISA.2. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 µg/ml (100 ?l/well) can bind Human CD16a. The EC50 of Human CD16a is 2.4 - 5.7 µg/ml.3. Labeling ratio of biotin to protein: 0.7-1.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR3A158F/V in the reference.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
25.1 kDa
AP Mol Mass:
48 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
