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Recombinant Human Carboxypeptidase E/CPE Protein (RPCB1438)

SKU:
RPCB1438
Size:
10µg
Tag:
C-hFc&His
Reactivity:
Human
Expression Host:
HEK293 cells
€169
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Description

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Product Name:Recombinant Human Carboxypeptidase E/CPE Protein
SKU:RPCB1438
Size:10µg
Tag:C-hFc&His
Reactivity:Human
Expression Host:HEK293 cells
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Human Carboxypeptidase E/CPE Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:<0.1EU/μg
Purity:> 85% by SDS-PAGE.
Formulation:Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:1363

Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P16870-1
Product ImageRecombinant Human Carboxypeptidase E/CPE Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 85-100kDa.

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