Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning ?-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
Product Name:
Carbonic Anhydrase XII/CA12 Recombinant Protein (RPES1735)
Product Code:
RPES1735
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CA12,CAXII,FLJ20151,HsT18816
Accession:
NP_001209.1
Sequence:
Met 1-Gln 291
Fusion tag:
C-His
Activity:
Measured by its esterase activity. The specific activity is >40 pmoles/min/µg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 µg enzyme at 400 nm in 100 ?L of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
31.6 kDa
AP Mol Mass:
40-45 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
