Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
Product Name:
Carbonic Anhydrase 10/CA10 Recombinant Protein (RPES1517)
Product Code:
RPES1517
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Carbonic Anhydrase-Related Protein 10, Carbonic Anhydrase-Related Protein X, CA-RP X, CARP X, Cerebral Protein 15, CA10,CA-RPX,CARPX,HUCEP-15
Accession:
NP_001076002.1
Sequence:
Met 1-Asn 300
Fusion tag:
C-His
Activity:
Measured by its esterase activity. The specific activity is >10 pmoles/min/µg, as measured with 1 mM 4-Nitrophenyl acetate and 5 µg enzyme at 400 nm in 100 ?L of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CARPX (NP_001076002.1) (Met 1-Asn 300) was expressed with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
33 kDa
AP Mol Mass:
37 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
