The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin, resulting in neuronal death. Whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF has a role in the negative regulation of neurotrophic actions in the brain. Human BDNF Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Immobilized Human pro-BDNF at 2ug/ml(100 ?l/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human pro-BDNF is 1.4 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human BDNF is produced by our E.coli expression system and the target gene encoding Ala19-Arg247 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
25.9 kDa
AP Mol Mass:
28 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 250mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
